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July 9, 2025

The Department of Transportation (DOT) now allows oral fluid testing as part of its drug testing regulations. This new option gives employers more flexibility while maintaining the integrity of the testing process. In this blog, we’ll explore what oral fluid testing entails, how it compares to traditional urine testing, and what employers and employees need to know about these changes.
One of the biggest highlights of the new oral fluid testing rule is that employers now have the option to decide which testing method to use.
Employers can:
Key Point: The flexibility applies across all DOT agencies (e.g., FMCSA, FAA, FTA, FRA, Coast Guard), making it a universal option under Part 40 regulations.
Oral fluid testing involves collecting saliva from the donor using a simple mouth swab. It’s scientifically backed as forensically defensible and viable, thanks to guidelines from the Department of Health and Human Services (HHS).
One notable difference between oral fluid and urine testing is the detection window:
Example: For post-accident or reasonable suspicion testing, oral fluid testing offers an immediate window of detection that may be preferable. However, for random or follow-up testing where patterns of drug use need to be identified, urine testing might still be the better option.

Important Note: Despite its shorter detection window, oral fluid testing is not a measure of impairment just like urine testing. Employers must avoid using test results to claim that an employee was "under the influence."
While oral fluid testing has its advantages, employers should also be aware of its limitations:
To incorporate oral fluid testing into their programs, employers should:


